全场光学相干层析在细胞成像中的应用研究

J4 ›› 2016, Vol. 33 ›› Issue (4): 437-443.

全场光学相干层析在细胞成像中的应用研究

胡斌江兴方石晓灏刘磊邓林红

1 常州大学生物医学工程与健康科学研究院，江苏常州 213164； 2 常州大学数理学院，江苏常州 213164； 3 江苏省现代光学技术重点实验室，江苏苏州 215006

收稿日期:2015-04-14 修回日期:2015-05-06 出版日期:2016-07-28 发布日期:2016-07-28
通讯作者: 邓林红（1960-）四川人，博士，教授，从事生物医学光子学的研究。 E-mail:dlh@cczu.edu.cn

Application of Full-field Optical Coherence Tomography for Cell Imaging

Received:2015-04-14 Revised:2015-05-06 Published:2016-07-28 Online:2016-07-28

摘要/Abstract

摘要： 研制了一套基于Linnik干涉仪的全场光学相干层析（full-field optical coherence tomography, FF-OCT）系统，以对细胞进行层析成像。不同于其它全场OCT系统的成像方法，该系统采用了基于希尔伯特（Hilbert）变换的图像恢复算法，只需要两幅移相干涉图就可以恢复出样品的结构信息，即使移相出现误差，仍然能够得到样品层析图。系统的实测纵向分辨率达到了1.1 μm，实测横向分辨率达到了2.5 μm。通过对洋葱表皮细胞的初步成像实验，验证了该FF-OCT系统的可行性，为进一步实现高分辨率细胞成像奠定了基础。

Abstract: A Linnik type full-field optical coherence tomography (FF-OCT) system was developed for tomographic imaging of biological cells. Different from other imaging methods used in FF-OCT systems, an image restoration method based on Hilbert transform required only two interference images was implemented which allowed the inaccurate phase-shift in experiments. The axial and lateral resolution of this system was 1.1 μm and 2.5 μm, respectively. Using this system, we obtained tomographic images of onion epithelial cells, thus confirming the feasibility of the proposed scheme and providing the foundation for further development of high-resolution system for cell imaging.

中图分类号:

O436.1

胡斌江兴方石晓灏刘磊邓林红. 全场光学相干层析在细胞成像中的应用研究[J]. J4, 2016, 33(4): 437-443.

参考文献

[1]Huang D, Swanson E A, Lin C P, et al.Optical coherence tomography[J].Science, 1991, 254(5035):1178-1181 [2]Balas C.Review of biomedical optical imaging—a powerful,non-invasive,non-ionizing technology for improving in vivo diagnosis[J].Measurement Science and Technology, 2009, 20(10):104020-104031 [3]Mclaughlin R A, Sampson D D.Clinical applications of fiber-optic probes in optical coherence tomography[J].Optical Fiber Technology, 2010, 16(6):467-475 [4]Peter B Noble, Robert A McLaughlin, Adrian R West, et al..Distribution of airway narrowing responses across generations and at branching points, assessed in vitro by anatomical optical coherence tomography[J].Respiratory Research, 2010, 11(9):1-12 [5]Hanna N, Saltzman D, Mukai D, et al.Two-dimensional and 3-dimensional optical coherence tomographic imaging of the airway,lung,and pleura[J].The Journal of thoracic and cardiovascular surgery, 2005, 129(3):615-622 [6]Izatt J A, Kulkarni M D, Yazdanfar S, et al.In vivo bidirectional color Doppler flow imaging of picoliter blood volumes using optical coherence tomography[J].Optics letters, 1997, 22(18):1439-1441 [7]Yu Xiaofeng, Ding Zhihua, Chen Yuheng, et al.Functional Expansion of Optical Coherence Tomography for Doppler Imaging[J].光学学报, 2006, 26(11):1717-1720 [8]Morgner U, Drexler W, K?rtner F, et al.Spectroscopic optical coherence tomography[J].Optics letters, 2000, 25(2):111-113 [9]Hee M R, Swanson E A, Fujimoto J G, et al.Polarization-sensitive low-coherence reflectometer for birefringence characterization and ranging[J].JOSA B, 1992, 9(6):903-908 [10]Fercher A F.Optical coherence tomography[J].Journal of Biomedical Optics, 1996, 1(2):157-173 [11]Wu Tong, Ding Zhihua.Development of 20 kHz Swept Source Optical Coherence Tomography System[J].中国激光, 2009, 36(2):503-508 [12]Beaurepaire E, Boccara A C, Lebec M, et al.Full-field optical coherence microscopy[J].Optics letters, 1998, 23(4):244-246 [13]Laude B, De Martino A, Drevillon B, et al.Full-field optical coherence tomography with thermal light[J].Applied Optics, 2002, 41(31):6637-6645 [14]Fornaro G, Franceschetti G, Lanari R, et al.Robust phase-unwrapping techniques: a comparison[J].JOSA A, 1996, 13(12):2355-2366 [15]Na J, Choi W J, Choi E S, et al.Image restoration method based on Hilbert transform for full-field optical coherence tomography[J].Applied Optics, 2008, 47(3):459-466 [16]Pavli?ek P, Michálek V.White-light interferometry—envelope detection by Hilbert transform and influence of noise[J].Optics and Lasers in Engineering, 2012, 50(8):1063-1068 [17]Novak J.Five-step phase-shifting algorithms with unknown values of phase shift[J].Optik-International Journal for Light and Electron Optics, 2003, 114(2):63-68 [18]Yang Yaliang.Full Field Optical Coherence Tomography[D]. Hangzhou: Zhejiang University, 2008 (in Chinese). [19]Zhu Yue, Gao Wanrong.High-resolution Full-Field Optical Coherence Tomography for Biological Tissue[J].中国激光, 2014, 41(8):114-121 [20]Zheng Jinggao.Investigation of Full-Field OCT and Morphological Study of Early Mouse Embryo Dveelopment[D]. Beijin: Tsinghua University, 2013 (in Chinese).