J4 ›› 2018, Vol. 35 ›› Issue (4): 395-401.

• 光谱 • 上一篇    下一篇

微弱荧光信号高灵敏大动态范围检测技术及应用

董 鸣，殷高方，赵南京，覃志松，赵 山，马明俊，肖 雪   

  1. 1中国科学院安徽光学精密机械研究所， 中国科学院环境光学与技术重点实验室，安徽 合肥 230031； 2中国科学技术大学，安徽 合肥 230026； 3安徽省环境光学检测技术重点实验室，安徽 合肥 230031
  • 收稿日期:2017-03-22 修回日期:2017-05-16 出版日期:2018-07-28 发布日期:2018-07-12
  • 通讯作者: 赵南京（1976-），博士，研究员，博士生导师，主要从事环境污染物光谱检测技术与方法研究。 E-mail:njzhao@aiofm.ac.cn
  • 作者简介:董 鸣(1991-)，研究生，研究方向为水体有机物荧光光谱检测技术。E-mail：mdong@aiofm.ac.cn
  • 基金资助:

    Supported by National Key Research and Development Program of China (国家重点研发计划项目，2016YFC1400600), National Natural Science Foundation of China （国家自然科学基金, 61378041, 31400317）, Anhui Science and Technology Major Project (安徽省科技重大专项, 15CZZ04125)，Science and Technology Service Network Initiative （中科院STS计划项目，KFJ-SW-STS-170）

High sensitivity and large dynamic range detection technology of weak fluorescence signal and its application

DONG Ming, YIN Gaofang, ZHAO Nanjing, QIN Zhisong, ZHAO Shan, MA Mingjun, XIAO Xue   

  1. 1Key Laboratory of Environmental Optics and Technology , Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China; 2 University of Science and Technology of China, Hefei 230026, China; 3 Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei 230031, China
  • Received:2017-03-22 Revised:2017-05-16 Published:2018-07-28 Online:2018-07-12

摘要：

针对水体有机物离散三维荧光光谱测量，设计了基于可变增益与积分放大技术的微弱荧光信号高灵敏、大动态范围检测电路和控制软件，进行了实验验证并应用于水体有机物离散三维荧光在线测量系统。在电路设计中，根据输入信号强度，自动程控CMOS多路模拟开关实现了前置增益放大器的调节，基于开关型电荷积分器实现了微弱脉冲荧光信号的同步积分放大。结果表明所设计电路最低检测电流为0.09 μA，动态检测范围为0.09 μA ~ 0.21 mA，检测限提高79.25倍。在水体有机物离散三维荧光测量系统的应用中，保证了系统的检测灵敏度，并提高了荧光信号的动态检测范围，实现了水体有机物的高精度测量。

关键词: 微弱荧光信号；可变增益；同步积分；水体有机物

Abstract:

According to the discrete three-dimensional (3D) fluorescence spectrum detection of organic matter in water, the high sensitivity and large dynamic range detection circuit and control software of weak fluorescence signal based on the variable gain and integral amplification technology are designed. Experiment verification is carried out and it’s applied to the on-line detection system for discrete 3D fluorescence of organic matter in water. In the designed circuit, according to the input signal intensity, automatic program control CMOS multi-channel analog switch is used to realize the adjustment of front gain amplifier. Based on the switching charge integrator, the synchronous integral amplification of weak pulse fluorescence signal is realized. Results show that the minimum detection current of the designed circuit is 0.09 μA, dynamic detection range is 0.09 μA ~ 0.21 mA, the detection limit is increased by 79.25 times. In the application of discrete 3D fluorescence detection system of organic matter in water, the system detection sensitivity is guaranteed, and dynamic detection range of fluorescence signal is improved. The high precision measurement of organic matter in water is realized.

Key words: weak fluorescence signal; variable gain; synchronous integral; organic matter in water

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