多种荧光物质重叠荧光光谱特征提取方法

doi:10.3969/j.issn.1007-5461.2026.02.009

量子电子学报 ›› 2026, Vol. 43 ›› Issue (2): 266-274.doi: 10.3969/j.issn.1007-5461.2026.02.009

多种荧光物质重叠荧光光谱特征提取方法

朱国庆 1, 王贻坤 2, 刘勇 2, 杨瑞芳 3*, 张洋 2*

1 安徽大学物质科学与信息技术研究院, 安徽合肥 230601; 2 中国科学院合肥物质科学研究院安徽光学精密机械研究所, 安徽省生物医学光学仪器工程技术研究中心, 安徽省医用光学诊疗技术与装备工程技术中心, 安徽合肥 230031; 3 中国科学院合肥物质科学研究院安徽光学精密机械研究所, 中国科学院环境光学与技术重点实验室, 安徽合肥 230031

收稿日期:2023-04-17 修回日期:2023-05-12 出版日期:2026-03-28 发布日期:2026-03-28
通讯作者: E-mail: rfyang@aiofm.ac.cn; yangz@aiofm.ac.cn E-mail:E-mail: rfyang@aiofm.ac.cn; yangz@aiofm.ac.cn
作者简介: 朱国庆 ( 1995 - ), 安徽淮北人, 研究生, 主要从事重叠荧光光谱解析方面的研究。E-mail: 1805811919@qq.com
基金资助:
国家自然科学基金 (62205345), 中国科学院合肥物质科学研究院院长基金 (YZJJZX202009, YZJJ2021GCH04), 安徽省药品监督管理局科技创新项目 (AHYJ-KJ-202115), 安徽省科技重大专项 (202103a07020008), 皖江新兴产业技术发展中心项目 (WJ21CYHXM07)

Extraction method of overlapping fluorescence spectral features of various fluorescent substances

ZHU Guoqing 1 , WANG Yikun 2 , LIU Yong 2 , YANG Ruifang 3*, ZHANG Yang 2*

1 Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, China; 2 Anhui Institute of Optics and Fine Mechanics, HFIPS, Chinese Academy of Sciences, Anhui Provincial Engineering Technology Research Center for Biomedical Optical Instrument, Anhui Provincial Engineering Technology Center for Medical Optical Diagnosis Treatment Technology and Instrument, Hefei 230031, China; 3 Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, HFIPS, Chinese Academy of Sciences, Hefei 230031, China

Received:2023-04-17 Revised:2023-05-12 Published:2026-03-28 Online:2026-03-28

摘要/Abstract

摘要： 皮肤自体荧光是由组织内多种内源性荧光物质在被激发后所产生的相互叠加的荧光, 可预示相关疾病的发生。然而, 内源性荧光团之间的光谱重叠会对目标检测造成干扰, 因此选择合适的检测光波长有助于提高识别效果。本研究选取晚期糖基化终末产物(AGEs)、还原型烟酰胺腺嘌呤二核苷酸(NADH)、黄素腺嘌呤二核苷酸(FAD)以及胶原蛋白(COL)作为实验对象, 首先构建了三维荧光光谱数据库。随后, 通过区域积分法提取了四种荧光物质的特征区域, 并结合无信息变量消除法进一步筛选出178个特征波长。最后, 基于多元线性回归建立校正曲线, 计算了各荧光物质的回收率。结果表明, AGEs、NADH、FAD和COL的回收率范围分别为70%~110%、80%~110%、70%~130%和50%~190%, 基于178个特征波长建立的模型对AGEs、NADH和FAD的预测效果较好, 实现了对重叠光谱中此三种荧光物质的高选择性波长筛选。

关键词: 生物医学光学, 特征荧光, 三维荧光光谱, 晚期糖基化终末产物

Abstract: Skin autofluorescence arises from the superposition of various endogenous fluorophores in tissues upon excitation, serving as an indicator of related disease states. However, spectral overlap among endogenous fluorophores complicates the detection of specific targets, so selecting optimal detection wavelengths can enhance the identification effectiveness of these targets. Advanced glycation end products (AGEs), reduced niacinamide adenine dinucleotide (NADH), flavin adenine dinucleotide (FAD) and collagen (COL) were selected as experimental subjects in this work. Firstly, a database of overlapping three-dimensional fluorescence spectra of the four fluorescent substances were constructed. Then, the characteristic regions of the four fluorescent substances were identified using the region integration method, and 178 characteristic wavelengths were selected through the elimination of uninformative variables. Finally, a calibration curve was established based on multiple linear regression, and the recovery rates of each fluorescent substance were calculated. The results showed that the recovery rates of AGEs, NADH, FAD, and COL were 70%–110%, 80%–110%, 70%–130%, and 50%–190%, respectively, and the predictive accuracy for AGEs, NADH, and FAD of the model based on 178 characteristic wavelengths was acceptable, achieving highly selective screening of detection wavelengths for the three fluorescent substances in overlapping spectra.

Key words: biomedical optics, characteristic fluorescence, three-dimensional fluorescence spectrum, advanced glycation end products

中图分类号:

R318.51

朱国庆 , 王贻坤 , 刘勇 , 杨瑞芳 , 张洋 . 多种荧光物质重叠荧光光谱特征提取方法[J]. 量子电子学报, 2026, 43(2): 266-274.

ZHU Guoqing , WANG Yikun , LIU Yong , YANG Ruifang , ZHANG Yang . Extraction method of overlapping fluorescence spectral features of various fluorescent substances[J]. Chinese Journal of Quantum Electronics, 2026, 43(2): 266-274.

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多种荧光物质重叠荧光光谱特征提取方法

Extraction method of overlapping fluorescence spectral features of various fluorescent substances

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