TDLAS技术在微生物（生长）检测领域研究进展

摘要/Abstract

摘要： 微生物检测在医疗诊断、食品安全、发酵工程、微生物科学研究等方面具有重要意义。微生物检测主要体现两个方面：微生物的判断和微生物生长曲线的测量。微生物的生长通常伴随着一系列与生长量相平行的DNA、酸碱度等生理指标和CO2、光学厚度等产物指标，这些指标为微生物检测提供了测量途径。其中，通过检测微生物代谢产物CO2，不仅可以排除死菌带来的干扰，而且能够实现快速、全自动检测，成为微生物检测的主要途径之一。TDLAS技术以其在测量CO2的高灵敏度高、结构简单、技术成熟等优势，为微生物检测提供一种快速、非侵入的新检测途径，对推动微生物检测技术的发展具有重要的意义。近几年TDLAS技术在微生物检测领域的发展取得巨大进展。

关键词: 光谱学, 可调谐半导体二极管激光吸收光谱技术, 微生物检测, 血培养, CO2检测

Abstract: Microbial monitoring plays a key role in a wide range of fields spanning from medical diagnosis and food safety to fermentation and microbiological research. Microbial monitoring mainly include two aspects: the judgment of microorganisms and the measurement of the growth curve of microorganisms. Microbial growth usually correlate directly with certain indicators, such as DNA, pH, CO2 and OD, which provide a way for microbial monitoring. Among them, CO2 measurements have become the preferred approach because they prevent the interference of the dead bacteria and provide a more rapid and automatic answer. TDLAS technology has demonstrated high sensitivity, while being a simple yet mature technological structure for the detection of CO2 to provide a rapid and non-intrusive technique for microbial monitoring. It is of great significance to promote a new approach for monitor microorganisms. TDLAS technology has made great progress in the field of microbial monitoring in recent years.

Key words: spectroscopy, Tunable Diode Laser Absorption Spectroscopy technology, microbial monitoring, blood culture, CO2 detection

付美娟1,张逸彪2，黄瑜倩1，邵杰1*. TDLAS技术在微生物（生长）检测领域研究进展[J]. 量子电子学报.

1Fu Meijuan, 2Zhang Yibiao,1Huang Yuqian, 1*Shao Jie. The Research Progress about Microbial Monitoring(Growth) based on TDLAS Technology[J]. Chinese Journal of Quantum Electronics.

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