激光吸收光谱技术在大肠杆菌生长测量中的应用

doi:10.3969/j.issn.1007-5461.2021.05.013

量子电子学报 ›› 2021, Vol. 38 ›› Issue (5): 684-690.doi: 10.3969/j.issn.1007-5461.2021.05.013

• “激光光谱新技术与应用”专辑 • 上一篇下一篇

激光吸收光谱技术在大肠杆菌生长测量中的应用

卢俊城1,2, 杨朝凤1, 管祖光3, 陈达如3, 邵杰1,2∗

1 浙江师范大学信息光学研究所, 浙江金华 321004; 2 瑞谱医疗设备(东莞)有限公司, 广东东莞 523808; 3 浙江师范大学杭州高等研究院, 浙江杭州 311200

收稿日期:2020-12-22 修回日期:2021-03-07 出版日期:2021-09-28 发布日期:2021-09-28
通讯作者: E-mail: shaojie@zjnu.cn E-mail:shaojie@zjnu.cn
作者简介:卢俊城 ( 1995 - ), 广东韶关人, 研究生, 主要从事 TDLAS 技术及应用方面的研究。 E-mail: lujuncheng@zjnu.edu.cn
基金资助:
Supported by National Natural Science Foundation of China (国家自然科学基金, 61775197), Dongguan Introduction Program of Leading Innovative and Entrepreneurial Talents (东莞市引进创新创业领军人才计划资助, 202023608)

Application of laser absorption spectroscopy in Escherichia coli growth measurement

LU Juncheng1,2, YANG Chaofeng1, GUAN Zuguang3, CHEN Daru3, SHAO Jie1,2∗

1 Institute of Information Optics, Zhejiang Normal University, Jinhua 321004, China; 2 Ruipu Medical Equipment (Dongguan) Co., Ltd., Dongguan 523808, China; 3 Hangzhou Institute of Advanced Studies, Zhejiang Normal University, Hangzhou 311200, China

Received:2020-12-22 Revised:2021-03-07 Published:2021-09-28 Online:2021-09-28

摘要/Abstract

摘要： 将高灵敏的激光吸收光谱技术应用于微生物生长测量领域, 实现了对以大肠杆菌为例的微生物生长过程实时监测和生长曲线的绘制。设计并搭建了一套实验测量装置, 对大肠杆菌生长过程中新陈代谢产生的 CO2 的二次谐波信号进行实时监测并拟合, 测量获取了大肠杆菌在 25、 26、 29、 32、 34、 37、 43 ◦C 培养条件下的生长曲线。实验结果表明测得的生长曲线能够准确反映大肠杆菌在不同温度下生长调整期、对数期、稳定期及衰亡期的时间, 证明了基于激光吸收光谱技术的微生物生长代谢产物 CO2 法可以很好地应用于微生物生长测量领域。

关键词: 光谱学, 微生物生长测量, 激光吸收光谱, 生长曲线, Gompertz 模型

Abstract: The highly sensitive laser absorption spectroscopy technology is applied to the field of microbial growth measurement to realize the real-time monitoring of the microbial growth process and the drawing of growth curves taking Escherichia coli as an example. A set of experimental measurement equipment is designed and built to monitor and fit the second harmonic signal of carbon dioxide (CO2) generated by metabolism of Escherichia coli in real time. The growth curves of E. coli are measured at 25, 26, 29, 32, 34, 37 and 43 ◦C. The experimental results show that the measured growth curve can accurately reflect the adjustment period, logarithmic phase, stable period and decay period of E. coli growth at different temperatures, which proves that the microbial growth metabolite (CO2) method based on laser absorption spectroscopy technology can be well applied to the field of microbial growth measurement.

Key words: spectroscopy, microbiological growth measurement, laser absorption spectroscopy, growth curve, Gompertz model

中图分类号:

O433.1

卢俊城, 杨朝凤, 管祖光, 陈达如, 邵杰, ∗. 激光吸收光谱技术在大肠杆菌生长测量中的应用[J]. 量子电子学报, 2021, 38(5): 684-690.

LU Juncheng, YANG Chaofeng, GUAN Zuguang, CHEN Daru, SHAO Jie, ∗. Application of laser absorption spectroscopy in Escherichia coli growth measurement[J]. Chinese Journal of Quantum Electronics, 2021, 38(5): 684-690.

参考文献

[1] J. B. Hughes, et al. Counting the uncountable: statistical approaches to estimating microbial diversity[J]. Applied and environmental microbiology, 2001, 67:4399-406.
[2] Pace N R . A Molecular View of Microbial Diversity and the Biosphere[J]. Science, 1997, 276:734-40.
[3] Ren Kexin, Chen Lanying, Bai Zhiyong, et al. Summary of microbial degradation of soil pesticides [J]. Rural practical technology, 2020(11):97-98.
任可心，陈兰英，白志勇，等. 土壤农药微生物降解综述 [J]. 农村实用技术，2020(11):97-98.
[4] Zhong Chongbin, Yu Hai, Feng Shu, et al. Microbial Co-Culture and Sewage Purification [J]. Journal of Microbiology, 2001，21(001):41-43(in Chinese).
仲崇斌，于海，冯树，等. 微生物共培养与污水净化 [J]. 微生物学杂志，2001，21(001):41-43.
[5] Ding Jiawei, Su Xinyao, Yin Xinxiang, et al. Study on Microbial Fermentation of Artemisia annua Leaves and Leaf Residues[J]. Biotechnology Bulletin, 2021，37(1):1-8(in Chinese).
丁嘉伟，苏新堯，尹薪项，等. 微生物发酵青蒿叶和叶渣的研究 [J]. 生物技术通报，2021，37(1):1-8
[6] Li Gaoxiang. The rise of enzyme engineering in China[J]. Chinese Journal of Biotechnology, 2015，31(06):805-819.
黎高翔. 中国酶工程的兴旺与崛起 [J]. 生物工程学报，2015，31(06):805-819.
[7] Gibson A M , Bratchell N , Roberts T A . Predicting microbial growth: growth responses of salmonellae in a laboratory medium as affected by pH, sodium chloride and storage temperature[J]. International Journal of Food Microbiology, 1988, 6(2):155-178.
[8] Song Sijia, Huang Ziyu, Lin Yingying, et al. Microbial Community Diversity in Egg Curds with Different Shelf Life Revealed by Illumina MiSeq Sequencing[J]. Food and Fermentation Industries, 1-8
宋思家，黄子彧，林莹莹，等. 基于Illumina MiSeq技术分析不同保质期鸡蛋干的微生物群落多样性 [J]. 食品与发酵工业:1-8
[9] Zhu Yanlei. Experimental Method of Bacteria Growth Curve Determination[J]. Journal of Microbiology, 2016,36(05):108-112.
朱艳蕾. 细菌生长曲线测定实验方法的研究 [J]. 微生物学杂志，2016，36(05):108-112.
[10] Vardanian A , Kurzbaum E , Farber Y , et al. Facilitated enumeration of the silicate bacterium Paenibacillus mucilaginosus comb. nov. (formerly Bacillus mucilaginosus) via tetrazolium chloride incorporation into a double agar-based solid growth medium[J]. Folia Microbiologica, 2018, 63(3): 401-404.
[11] Chen Yuqi. Comparison of growth measurement methods in microbial growth curves[J]. Light Industry Science and Technology, 2020, 36(04):7-8+12.
陈玉琪. 微生物生长曲线中生长量测定方法的比较 [J]. 轻工科技，2020，36(04):7-8+12.
[12] Fiach O’Mahony, Green R A , Baylis C , et al. Analysis of total aerobic viable counts in samples of raw meat using fluorescence-based probe and oxygen consumption assay[J]. Food Control, 2009, 20(2):129-135.
[13] Rice J , Cooper C , Steiner B , et al. Validation of the Soleris?? NF-TVC Method for Determination of Total Viable Count in a Variety of Foods[J]. journal of aoac international, 2013, 96(2).
[14] Allen M G . Diode Laser Absorption Sensors for Gas-Dynamic and Combustion Flows[J]. Measurement ence and Technology, 1998, 9(4):545-562.
[15] Ballester J , Garcia-Armingol T . Diagnostic techniques for the monitoring and control of practical flames[J]. Progress in Energy & Combustion ence, 2010, 36(4):375-411.
[16] Rea , E C , Hanson R K . Rapid laser-wavelength modulation spectroscopy used as a fast temperature measurement technique in hydrocarbon combustion[J]. Applied Optics, 1988, 27(21):4454-64.
[17] Kluczynski P , Gustafsson J , Lindberg S M , et al. Wavelength modulation absorption spectrometry — an extensive scrutiny of the generation of signals[J]. Spectrochimica Acta Part B Atomic Spectroscopy, 2001, 56(8):1277-1354.
[18] Huang L . Simulation and evaluation of different statistical functions for describing lag time distributions of a bacterial growth curve[J]. Microbial Risk Analysis, 2016, 1:47-55.
[19] M. Li, et al. Comparison of Mathematical Models of Lactic Acid Bacteria Growth in Vacuum‐Packaged Raw Beef Stored at Different Temperatures[J]. Journal of Food Science, 2014, 78(4):M600-M604.

激光吸收光谱技术在大肠杆菌生长测量中的应用

Application of laser absorption spectroscopy in Escherichia coli growth measurement

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