J4 ›› 2014, Vol. 31 ›› Issue (1): 12-17.

• 光谱 • 上一篇    下一篇

几种人工合成色素的荧光光谱与拉曼光谱研究

逯美红,雷海英,王志军,吴巧凤   

  1. 1 长治学院电子信息与物理系, 山西 长治 046011; 2 长治学院生物科学与技术系, 山西 长治 046011; 3 西北工业大学理学院, 陕西 西安710129
  • 收稿日期:2013-03-29 修回日期:2013-07-24 出版日期:2014-01-28 发布日期:2013-12-31
  • 通讯作者: 逯美红(1979—),女,山西孝义人,硕士,讲师,从事光谱技术方面的研究。 E-mail:lmhxueer@126.com
  • 基金资助:
    山西省高校科技开发项目资助(2010128)

Fluorescence spectra and Raman spectra of several synthetic food colors

LU Mei-hong, LEI Hai-ying, WANG Zhi-jun, WU Qiao-feng   

  1. 1 Department of Electronic information and Physics, Changzhi University, Shanxi 046011, China; 2 Department of Biological sciences and Technology Changzhi University, Shanxi 046011, China; 3 Department of Physics, Northwestern Polytechnical University, Xi’an 710129, China
  • Received:2013-03-29 Revised:2013-07-24 Published:2014-01-28 Online:2013-12-31

摘要: 应用荧光光谱仪及微拉曼光谱仪分别对几种常见的人工合成色素溶液的荧光光谱和拉曼光谱进行了实验研究。一方面,首次测量其不同浓度合成色素溶液的荧光光谱,讨论其谱线特性。另一方面,以有致癌性的合成色素苋菜红为例,应用拉曼光谱技术进行定性和定量检测,验证拉曼光谱对色素检测的可行性。为了提高探测灵敏度,以55nm的金纳米粒子溶胶为基底,在785nm激发光下对不同浓度的苋菜红溶液进行了表面增强拉曼光谱探测,探测到的最低浓度为10-17mol/L。分析结果为这种色素的合理利用及安全快速检测提供了有效的光谱实验依据,也为其它色素的检测提供了参考方法。

关键词: 光谱学, 荧光光谱, 表面增强拉曼光谱, 苋菜红

Abstract: Fluorescence spectrometer and confocalmicro-Raman spectrometer were used to study the fluorescence spectroscopy and Raman spectroscopy of several synthetic food Colors. On the one hand, the fluorescence spectra of different concentrations were first measured and the spectral characteristics were discussed. On the other hand, the Raman spectroscopy of carcinogenic organic amaranth sample was studied as an example. In order to improve the detection sensitivity, 55nm gold nanparticles collosol as substrate and 785nm excitation light were used to study the surface enhanced Raman scattering spectrum of amaranth with different concentrations, and the detected minimum concentration was 10-17mol/L. The results provide spectrum experiment basis for reasonable utilization of the artificial color and the safe and rapid detection, also provides a new method for safety testing of other artificial color.

Key words: spectroscopy, fluorescence spectroscopy, surface enhanced Raman scattering spectrum, amaranth

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